barcoded sindbis virus library Search Results


sindbis viral library abarcoded sindbis virus library  (Addgene inc)
92
Addgene inc sindbis viral library abarcoded sindbis virus library
Figure 1. MAPseq and BARseq projection mapping of individual olfactory bulb neurons (A) Schematics of the MAPseq strategy which uses RNA barcodes to label neurons and map their brain-wide projections. (B) Infection of mitral and tufted cells by <t>Sindbis</t> virus carrying the barcodes and a fluorophore (EGFP). (C) Laser Capture Micro-Dissection of target brain regions from Nissl stained coronal sections registered to the Allen Brain reference atlas. (D) Illustration of laminar positions of mitral, tufted, and deep cells (left) and an example BARseq sequencing image of the barcoded cells (center). The first several bases of the barcode sequences in two example neurons analyzed via BARseq and their projection patterns across 6 bulb target regions (right). Scale bar = 100mm. (E) (Left) projection patterns (415 neurons, 2 mice) identified via BARseq and their soma locations relative to the mitral cell layer (MCL). Columns represent bulb projection target regions and rows indicate individual neurons. (right) Cell identities based on soma positions. Projection strength of each barcoded neuron has been normalized so that the maximum strength is 1 (row). (F) (Left) Soma positions of template neurons shown relative to MCL (y axis) and to glomerular layer (x axis) that were used to train the projection-based classifier. The sectioning planes are not necessarily perpendicular to the mitral cell layer, and thus the distances measured may be inflated. Neuronal identity (colors) is based on laminar positions (tufted, mitral, and deep cells). (Right) Classification confusion matrix of all three cell classes using the BARseq-based classifier versus position-defined classes. See also Figures S1 and S2.
Sindbis Viral Library Abarcoded Sindbis Virus Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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sindbis virus for mapseq  (Cold Spring Harbor Laboratory Meetings)
90
Cold Spring Harbor Laboratory Meetings sindbis virus for mapseq
Figure 1. MAPseq and BARseq projection mapping of individual olfactory bulb neurons (A) Schematics of the MAPseq strategy which uses RNA barcodes to label neurons and map their brain-wide projections. (B) Infection of mitral and tufted cells by <t>Sindbis</t> virus carrying the barcodes and a fluorophore (EGFP). (C) Laser Capture Micro-Dissection of target brain regions from Nissl stained coronal sections registered to the Allen Brain reference atlas. (D) Illustration of laminar positions of mitral, tufted, and deep cells (left) and an example BARseq sequencing image of the barcoded cells (center). The first several bases of the barcode sequences in two example neurons analyzed via BARseq and their projection patterns across 6 bulb target regions (right). Scale bar = 100mm. (E) (Left) projection patterns (415 neurons, 2 mice) identified via BARseq and their soma locations relative to the mitral cell layer (MCL). Columns represent bulb projection target regions and rows indicate individual neurons. (right) Cell identities based on soma positions. Projection strength of each barcoded neuron has been normalized so that the maximum strength is 1 (row). (F) (Left) Soma positions of template neurons shown relative to MCL (y axis) and to glomerular layer (x axis) that were used to train the projection-based classifier. The sectioning planes are not necessarily perpendicular to the mitral cell layer, and thus the distances measured may be inflated. Neuronal identity (colors) is based on laminar positions (tufted, mitral, and deep cells). (Right) Classification confusion matrix of all three cell classes using the BARseq-based classifier versus position-defined classes. See also Figures S1 and S2.
Sindbis Virus For Mapseq, supplied by Cold Spring Harbor Laboratory Meetings, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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barcoded sindbis virus  (Jackson Laboratory)
90
Jackson Laboratory barcoded sindbis virus
Figure 1. MAPseq and BARseq projection mapping of individual olfactory bulb neurons (A) Schematics of the MAPseq strategy which uses RNA barcodes to label neurons and map their brain-wide projections. (B) Infection of mitral and tufted cells by <t>Sindbis</t> virus carrying the barcodes and a fluorophore (EGFP). (C) Laser Capture Micro-Dissection of target brain regions from Nissl stained coronal sections registered to the Allen Brain reference atlas. (D) Illustration of laminar positions of mitral, tufted, and deep cells (left) and an example BARseq sequencing image of the barcoded cells (center). The first several bases of the barcode sequences in two example neurons analyzed via BARseq and their projection patterns across 6 bulb target regions (right). Scale bar = 100mm. (E) (Left) projection patterns (415 neurons, 2 mice) identified via BARseq and their soma locations relative to the mitral cell layer (MCL). Columns represent bulb projection target regions and rows indicate individual neurons. (right) Cell identities based on soma positions. Projection strength of each barcoded neuron has been normalized so that the maximum strength is 1 (row). (F) (Left) Soma positions of template neurons shown relative to MCL (y axis) and to glomerular layer (x axis) that were used to train the projection-based classifier. The sectioning planes are not necessarily perpendicular to the mitral cell layer, and thus the distances measured may be inflated. Neuronal identity (colors) is based on laminar positions (tufted, mitral, and deep cells). (Right) Classification confusion matrix of all three cell classes using the BARseq-based classifier versus position-defined classes. See also Figures S1 and S2.
Barcoded Sindbis Virus, supplied by Jackson Laboratory, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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virus strains sindbis virus for mapseq  (Addgene inc)
97
Addgene inc virus strains sindbis virus for mapseq
Figure 1. MAPseq and BARseq projection mapping of individual olfactory bulb neurons (A) Schematics of the MAPseq strategy which uses RNA barcodes to label neurons and map their brain-wide projections. (B) Infection of mitral and tufted cells by <t>Sindbis</t> virus carrying the barcodes and a fluorophore (EGFP). (C) Laser Capture Micro-Dissection of target brain regions from Nissl stained coronal sections registered to the Allen Brain reference atlas. (D) Illustration of laminar positions of mitral, tufted, and deep cells (left) and an example BARseq sequencing image of the barcoded cells (center). The first several bases of the barcode sequences in two example neurons analyzed via BARseq and their projection patterns across 6 bulb target regions (right). Scale bar = 100mm. (E) (Left) projection patterns (415 neurons, 2 mice) identified via BARseq and their soma locations relative to the mitral cell layer (MCL). Columns represent bulb projection target regions and rows indicate individual neurons. (right) Cell identities based on soma positions. Projection strength of each barcoded neuron has been normalized so that the maximum strength is 1 (row). (F) (Left) Soma positions of template neurons shown relative to MCL (y axis) and to glomerular layer (x axis) that were used to train the projection-based classifier. The sectioning planes are not necessarily perpendicular to the mitral cell layer, and thus the distances measured may be inflated. Neuronal identity (colors) is based on laminar positions (tufted, mitral, and deep cells). (Right) Classification confusion matrix of all three cell classes using the BARseq-based classifier versus position-defined classes. See also Figures S1 and S2.
Virus Strains Sindbis Virus For Mapseq, supplied by Addgene inc, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Figure 1. MAPseq and BARseq projection mapping of individual olfactory bulb neurons (A) Schematics of the MAPseq strategy which uses RNA barcodes to label neurons and map their brain-wide projections. (B) Infection of mitral and tufted cells by Sindbis virus carrying the barcodes and a fluorophore (EGFP). (C) Laser Capture Micro-Dissection of target brain regions from Nissl stained coronal sections registered to the Allen Brain reference atlas. (D) Illustration of laminar positions of mitral, tufted, and deep cells (left) and an example BARseq sequencing image of the barcoded cells (center). The first several bases of the barcode sequences in two example neurons analyzed via BARseq and their projection patterns across 6 bulb target regions (right). Scale bar = 100mm. (E) (Left) projection patterns (415 neurons, 2 mice) identified via BARseq and their soma locations relative to the mitral cell layer (MCL). Columns represent bulb projection target regions and rows indicate individual neurons. (right) Cell identities based on soma positions. Projection strength of each barcoded neuron has been normalized so that the maximum strength is 1 (row). (F) (Left) Soma positions of template neurons shown relative to MCL (y axis) and to glomerular layer (x axis) that were used to train the projection-based classifier. The sectioning planes are not necessarily perpendicular to the mitral cell layer, and thus the distances measured may be inflated. Neuronal identity (colors) is based on laminar positions (tufted, mitral, and deep cells). (Right) Classification confusion matrix of all three cell classes using the BARseq-based classifier versus position-defined classes. See also Figures S1 and S2.

Journal: Cell

Article Title: High-throughput sequencing of single neuron projections reveals spatial organization in the olfactory cortex.

doi: 10.1016/j.cell.2022.09.038

Figure Lengend Snippet: Figure 1. MAPseq and BARseq projection mapping of individual olfactory bulb neurons (A) Schematics of the MAPseq strategy which uses RNA barcodes to label neurons and map their brain-wide projections. (B) Infection of mitral and tufted cells by Sindbis virus carrying the barcodes and a fluorophore (EGFP). (C) Laser Capture Micro-Dissection of target brain regions from Nissl stained coronal sections registered to the Allen Brain reference atlas. (D) Illustration of laminar positions of mitral, tufted, and deep cells (left) and an example BARseq sequencing image of the barcoded cells (center). The first several bases of the barcode sequences in two example neurons analyzed via BARseq and their projection patterns across 6 bulb target regions (right). Scale bar = 100mm. (E) (Left) projection patterns (415 neurons, 2 mice) identified via BARseq and their soma locations relative to the mitral cell layer (MCL). Columns represent bulb projection target regions and rows indicate individual neurons. (right) Cell identities based on soma positions. Projection strength of each barcoded neuron has been normalized so that the maximum strength is 1 (row). (F) (Left) Soma positions of template neurons shown relative to MCL (y axis) and to glomerular layer (x axis) that were used to train the projection-based classifier. The sectioning planes are not necessarily perpendicular to the mitral cell layer, and thus the distances measured may be inflated. Neuronal identity (colors) is based on laminar positions (tufted, mitral, and deep cells). (Right) Classification confusion matrix of all three cell classes using the BARseq-based classifier versus position-defined classes. See also Figures S1 and S2.

Article Snippet: Barcoded Sindbis viral library Abarcoded Sindbis virus library (JK100L2, Addgene plasmid #79785) was generated and used forMAPseq andBARseq experiments as described previously (Chen et al., 2019; Han et al., 2018; Huang et al., 2020; Kebschull et al., 2016).

Techniques: Infection, Virus, Dissection, Staining, Sequencing